Preparation of Estradiol - HRP Conjugate and its Use in Different ELISA
Keywords:
Antibody capture assay, Competitive ELISA, Estradiol, HRPAbstract
In the present study estradiol was conjugated with HRP and polyclonal antibodies against estradiol, produced in rabbits. For the characterization of these antibodies and Estradiol-HRP conjugate, different immunoassays were performed mainly Competitive ELISA and Antibody Capture assay. Purification of Anti-Estradiol was done by ammonium salt precipitation and ion exchange chromatography. Preparation of enzyme conjugate was done by conjugation of Estradiol 6-CMO with HRP. Various diagnostic assays were performed like checker board assay, Assay to determine enzyme-conjugate titer, Standard competitive ELISA assay, Antibody capture assay.
Downloads
Downloads
Published
Issue
Section
License
Copyright (c) 2020 Gaurav Kumar, Mukesh Kumar
This work is licensed under a Creative Commons Attribution 4.0 International License.