Preparation of Estradiol - HRP Conjugate and its Use in Different ELISA

Authors

  • Gaurav Kumar B.Tech., Department of Biotechnology, Ambala College of Engineering & Applied Research, Ambala, India
  • Mukesh Kumar Assistant Professor, Department of Biotechnology, Ambala College of Engineering & Applied Research, Ambala, India

Keywords:

Antibody capture assay, Competitive ELISA, Estradiol, HRP

Abstract

In the present study estradiol was conjugated with HRP and polyclonal antibodies against estradiol, produced in rabbits. For the characterization of these antibodies and Estradiol-HRP conjugate, different immunoassays were performed mainly Competitive ELISA and Antibody Capture assay. Purification of Anti-Estradiol was done by ammonium salt precipitation and ion exchange chromatography. Preparation of enzyme conjugate was done by conjugation of Estradiol 6-CMO with HRP. Various diagnostic assays were performed like checker board assay, Assay to determine enzyme-conjugate titer, Standard competitive ELISA assay, Antibody capture assay.

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Published

18-12-2020

How to Cite

[1]
G. Kumar and M. Kumar, “Preparation of Estradiol - HRP Conjugate and its Use in Different ELISA”, IJRESM, vol. 3, no. 12, pp. 64–67, Dec. 2020.

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