The Effect of Assisted Laser Hatching On the Clinical Pregnancy Rates, in Fresh and Frozen Embryo Transfer, in Infertile Women in Jalandhar, Punjab

Abstract

Post embryo transfer, in an IVF cycle, the transferred embryo has to expand and perforate the Zone Pellucida (ZP), so as to implant and lead to a successful pregnancy outcome. The zone pellucida is a glycoprotein sphere covering the blastocyst. The lysis of zona pellucida is called Zone Hatching. Sometimes the embryo is not able to hatch out from the zona pellucida leading to failed implantation. Assisted laser hatching may be a novel method to improve the implantation rates in patients undergoing Fresh or Frozen Embryo Transfer cycle. The aim of this study was to determine if Assisted Laser Hatching of the transferred embryos on Day # embryos, before embryo transfer could improve the clinical pregnancy rates in 100 infertile patients, undergoing Fresh or Frozen Embryo Transfer Cycle. We noted that Assisted Laser Hatching did not favour the outcome of occurrence of biochemical and clinical pregnancy (LAH v/s no LAH: 52% v/s 64%). Also the occurrence of multiple gestations (LAH v/s no LAH: 94.2% v/s 95.3%) was not affected by AH. In fact, the proportions of those who experienced adverse events were significantly higher (25 %) in the Laser Hatching Group.