Expressions of Rat Antibodies in the Serum of Laying Hens Repeatedly Immunized with Rattus rattus Meat Extracts: Potential Antibodies for Development of Kits in the Prevention of Meat Adulteration

Abstract

In the development of immunoassay-based diagnostics such as enzyme-linked immunosorbent assays (ELISA) and immunochromatography (IC), antibodies play a crucial role. In this study, we succeeded in producing antibodies against house rat (Rattus rattus) meat extract (RME) by immunizing adult laying hens using (RME) repeatedly. Adult laying hens were immunized four times, with a dose of 200 μg/head in the first injection, 100 μg/head in subsequent immunizations with a two-week interval between vaccinations. The results of ELISA analysis showed that the antibodies produced could be used to distinguish between rat meat and beef, goat, chicken, as well as pork (p < 0.0002). Therefore, it can be concluded that antibodies against rat meat extract produced in laying hens in this study have high potential as raw material for the development of kits for meat authentication between animal species. Further research is needed for application in the prevention of meat adulteration.