Qualitative and Quantitative Phytochemical Screening, TLC Fingerprinting, HPTLC, and Proximate Analysis of Premna serratifolia, a Medicinally Important Plant

Abstract

Premna serratifolia belongs to family labiateae. It is a medicinal plant which is traditionally used in Maharashtra for various ailments. The present study focuses on qualitative and quantitative determination of phytoconstituents which makes the plant to be a potential source of alternative medicine. Quantitative phytochemical analysis reveals the presence of flavanoids, and Phenolic compounds, saponins, cardiac glycosides and tannin in extracts using methanol, n-hexane and chloroform. Total flavanoids were found to be 0.64mg/ml using colorimetric method. Whereas total phenolic compound analysis estimated were 1.54mg/ml. The plant extracts have been subjected to TLC fingerprinting and HPTLC analysis for finding out the presence flavonoids. Flavonoids are hydroxylated phenolic substances synthesized by plants and they have been found to possess antimicrobial substances against wide variety of microorganisms in vitro. Essential oil extraction from the leaveswas done by steam distillation method and the oil was again subjected to antimicrobial activity. Proximate analysis of the plant material also was performed to find out the nutritional value. It can be concluded that Premna serratifolia is rich in phytochemicals and it showed antimicrobial activity. So the plant can be considered as a potential source of drugs.